Mutagenesis of a plastidial lysophosphatidic acid acyltransferase.

نویسندگان

  • S Maisonneuve
  • J J Bessoule
  • R Lessire
  • M Delseny
  • T J Roscoe
چکیده

A combination of site-directed and random mutagenesis generated sequence variants of a plastidial lysophosphatidic acid acyltransferase. Alanine substitutions of residues present within two conserved motifs including the putative catalytic histidine resulted in a loss of acyltransferase activity assessed as complementation competence. Substitutions at five sites within the central core resulted in reduced or loss of activity. Truncation mutants reveal that sequences in the C-terminal moiety are essential for function.

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عنوان ژورنال:
  • Biochemical Society transactions

دوره 28 6  شماره 

صفحات  -

تاریخ انتشار 2000